Establishment and preliminary application of the McA-RH7777/Buffalo hepatoma rat model with bioluminescence imaging
10.3760/cma.j.issn.1007-8118.2015.05.013
- VernacularTitle:联合生物素荧光成像建立McA-RH7777/Buffalo大鼠肝癌模型及其初步应用
- Author:
Lichao PAN
;
Aiqun ZHANG
;
Jia BAI
;
Xiaoyu TAN
;
Xinlan GE
;
Yanhua LAI
;
Jiahong DONG
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Hepatoma Model;
Bioluminescence imaging
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(5):332-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a luciferase labeled McA-RH7777 hepatoma rat model,which could be used for gross observation to further observe the effect of selective ligation of the portal vein and bile duct on tumor growth and metastasis.Methods The luciferase gene was transfected into rat McA-RH7777 hepatoma cells with pCDH-puromycin-CMV as the carrier,which were subcutaneously inoculated into Buffalo rats.Tumor pieces were then heterotransplanted into the left lateral lobe of the allogenic rat liver to observe the tumor growth in vivo.After the successful hepatoma modeling,the rats were randomly divided into three groups,namely the implanted portal vein group with combined portal vein and bile duct ligation,the implanted portal vein group with single portal vein ligation and sham operation group.The rats were executed at the 1 st week and 2nd week after ligation,and the livers were dissected to record the tumor growth and metastasis inside and outside the liver,respectively.Results The tumor formation rates of Buffalo rats after subcutaneous and intrahepatic implantation were both 100％.The fluorescence signal implanted into the liver lobe could be observed in vivo after the intrahepatic implantation of luciferase transfected Luc-McA-RH7777 at 2nd week,the range and intensity of which increased over time.Only local tumor growth could be found at the 4th week,without obvious intrahepatic and lung metastasis.However,both an increased in situ tumor volume and the pulmonary metastasis could be observed in the implanted portal vein group with combined portal vein and bile duct ligation at 2nd week after the ligation.Immunohistochemistry showed AFP positive immunoreactions in the vast majority of intrahepatic tumor cells and Luc positive immunoreactions in part of tumor cells.Conclusion Luc-McA-RH7777 cells could be used to establish the heptoma rat model and the in vivo analysis within the Buffalo rat liver demonstrated that the combined ligation of the portal vein and bile duct can accelerate the development and metastasis of liver cancer.
