Gene expressions of the human intrahepatic cholangiocarcinoma RBE cell line
10.3760/cma.j.issn.1007-8118.2015.06.004
- VernacularTitle:人肝内胆管癌细胞株RBE的基因表达
- Author:
Jie WANG
;
Jing XU
;
Jiefeng HE
;
Lingmin LI
;
Yanhong TAN
;
Xiuhua CHEN
;
Yanling HE
- Publication Type:Journal Article
- Keywords:
Intrahepatic cholangiocarcinoma;
Hepatic stem/progenitor cell;
Gene microarray;
Signaling pathway
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(6):373-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differentially expressed genes between the NCAM + c-Kit +RBE and NCAM-c-Kit-RBE of intrahepatic cholangiocarcinoma (ICC) cell lines,and to screen out the differentially expressed genes that are related to the stem cell signaling pathways.Methods Magnetic activated cell sorting was used to isolate the NCAM + c-Kit +/NCAM-c-Kit-subset cells,and then Agilent Whole Human Genome Microarray Kit was used to test the difference in gene expressions between the NCAM + cKit + and NCAM-c-Kit-subset cells.The difference in gene expressions related to the stem cell signaling pathways was analyzed by the SAS system.The result of the microarray was further confirmed by RT-PCR.Results The total differentially expressed genes which could be found through gene microarray were 7270 [foldchange(fc) ≥2 or fc ≤0.5].Compared with the NCAM-c-Kit-RBE,3572 genes were upregulated while 3698 genes were downregulated.The differences in gene expressions related to the stem cell signaling pathways were 421 (fc ≥2 or fc ≤ 0.5),among which 231 genes were upregulated while 190 genes were downregulated.Conclusions High-flux microarray could be used to screen out lots of differentially expressed genes between the NCAM + c-Kit + and NCAM-c-Kit-RBE cells.The differences in gene expression in the stem cell signaling pathways could also be further analyzed using the SAS system.
