Hepatic ischemia/reperfusion promote hematogenic metastasis of hepatocellular carcinoma in mice through induction of MMP-9 expression
10.3760/cma.j.issn.1007-8118.2014.11.012
- VernacularTitle:基质金属蛋白酶-9在小鼠肝脏部分缺血再灌注并肝细胞癌门静脉转移模型中的表达及临床意义
- Author:
Yi LIU
;
Jin WANG
;
Yuxin CHEN
;
Kesen XU
;
Hailin LI
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Ischemia/reperfusion;
Matrix metalloproteinase-9;
Portal vein metastasis
- From:
Chinese Journal of Hepatobiliary Surgery
2014;20(11):810-815
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ischemia/reperfusion(I/R) on tumor metastasis in a experimental mouse model of hematogenous metastasis after I/R; we also quantitated expression of matrix metalloproteinases-9 (MMP-9) during I/R.Methods An experimental mouse model of metastasis after partial hepatic I/R was designed to determine the effects of I/R on tumor metastasis to liver.Tumor loads were valued 7 days after operation.In addition,tissue analysis for alanine transaminase,aspartate transaminase (AST) and matrix metalloproteinases and 8 h reperfusion were performed.Results After 2 h hepatic reperfusion,ALT and AST in I 45 min group were higher than the sham group and I 30 min group (P ＜0.05).ALT and AST in the sham group were both a little higher than the normal.ALT and AST in the I 45 min group and I 30 min group at 8 h were both higher than those at 2 h reperfusion(P ＜0.05).The tumor load (valued by Hepatic replacement area) and the expression of MMP-9 in ischemic lobe in I 45 min group were greater than that after 30 min group and sham group.(P =0.013,P =0.007).There was no statistical difference on tumor load between the right lobe of sham operated mice and the right lobe (non-ischemic lobe) of mice subjected to I/R(P =0.089).Mouse survival were compared among the groups.Mice in Sham group lived longer than I 30 min group (P =0.041).And there were no statistical significance between I 45 min group and I 30 min group (P =0.055).MMP-9 expression in I 45 min group was higher than I 30 min group(P ＜ 0.001).Conclusion Hepatic I/R promotes liver hematogenic metastasis of hepatocellular carcinoma in mice through induction of MMP-9 expression.
