Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma CNE-2 cells

VernacularTitle:自噬活性的降低增加人鼻咽癌CNE-2细胞的放射敏感性
Author: Xiaodi LU ; Xiaodong ZHU ; Wei ZHAO ; Song QU ; Xia LIANG ; Fang SU
Publication Type:Journal Article
Keywords: ATG5; Autophagy; Nasopharyngeal carcinoma; Radiosensitivity
From: Chinese Journal of Radiological Medicine and Protection 2015;35(6):423-427
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of autophagy in radiosensitivity of nasopharyngeal carcinoma CNE-2 cells.Methods The expression of ATG5 in CNE-2 cells was inhibited by lentiviral mediated RNA interference.The cells were divided into three groups:control group,NC group and ATG5 group.Cell proliferation,apoptosis and radiosensitivity were detected by CCK-8 method,flow cytometry and colony-forming assay,respectively.Results Compared with the NC group and control group,the survival of ATG5-interfected cells was reduced (F =3.755,46.086,8.609,44.160,P ＜ 0.05).After 6 Gy X-ray irradiation,the apoptosis index of the ATG5 group significantly higher than that of NC group and control group (F =394.876,P ＜ 0.05).In addition,the radiosensitivity of ATG5 group was also higher than that of control cells.Conclusions Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma cells.