The protective effect of pyrrolidine dithiocarbamate on acute radiation injury in mice
10.3760/cma.j.issn.0254-5098.2015.04.005
- VernacularTitle:吡咯烷二硫代氨基甲酸盐对急性辐射损伤小鼠的防护作用
- Author:
Jin JIN
;
Jiali BAI
;
Wei LONG
;
Xiu SHEN
;
Wenqing XU
;
Zewei ZHOU
- Publication Type:Journal Article
- Keywords:
Pyrrolidine dithiocarbamate;
γ-rays;
ICR mice;
Radiation injury
- From:
Chinese Journal of Radiological Medicine and Protection
2015;35(4):257-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of pyrrolidine dithiocarbamate (PDTC) on acute irradiated mice.Methods The 6-8 weeks old male ICR mice were randomly divided into five groups:irradiation alone group (IR),positive control group (amifostine WR-2721 250 mg/kg) and PDTC of 30,60 and 90 mg/kg dose groups.Each group had 10 mice and the drug was given at 0.5 h before whole body irradiation.At 30 d post-irradiation of 7.5 Gy 137 Cs γrays,the mice survival were observed.At 8 d post-irradiation of 5.0 Gy 137 Cs γ-rays,the peripheral blood,hematopoietic system and organ indexes were observed to evaluate the radiation protective effect of PDTC.Results PDTC increased the 30-day survival rates and 60 mg/kg dose had the most obvious effect by increase the survival to 60％ (6/10).The survivals of irradiation alone group and the amifostine positive control group was 10％ (1/10) and 70％ (7/10),respectively.Compared with the irradiation alone group,60 mg/kg PDTC group had the significant difference in spleen index,WBC,HGB,PLT,bone marrow nucleated cells and colony forming unit of spleen (t =2.354,4.793,2.342,6.542,2.649,3.982,P ＜ 0.05).Conclusions PDTC is effective in radiation protection with an optimum dose of 60 mg/kg.
