Down regulation of miR-203 in radiation-induced thymic lymphoma promoted cells proliferation and inhibited apoptosis
10.3760/cma.j.issn.0254-5098.2015.01.005
- VernacularTitle:Down regulation of miR-203in radiation-induced thymic lymphoma promoted cells proliferation and inhibited apoptosis
- Author:
Chaoxiong ZHANG
;
Mingjian ZHANG
;
Fu GAO
;
Chuanfeng ZHOU
;
Pei ZHANG
;
Jianming CAI
;
Cong LIU
- Publication Type:Journal Article
- Keywords:
MiR-203;
Radiation-induced thymic lymphoma (RITL);
TANK-binding kinase 1 (TBK1);
SLUG (SNAI2);
Cyclin D1 (CCND1)
- From:
Chinese Journal of Radiological Medicine and Protection
2015;35(1):28-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of miR-203 in radiation-induced thymic lymphoma (RITL).Methods A 60Co irradiator was used for total-body irradiation.MicroRNAs(miRNAs) level was assayed by qRT-PCR.Cell proliferation was assayed by MTT assay.Cell apoptosis was examined by fluorescence activated cell sorter (FACS).Dual luciferase reporter assay system was used to detect the 3'UTR reporter.Results MiR-203 was down-regulated in RITL tissues.Overexpression of miR-203 strongly inhibited the proliferation of both NIH3T3 cells and EL4 cells and vice versa.MiR-203 inhibited cells proliferation and induced apoptosis via TANK-binding kinase (TBK1),SLUG (SNAI2) and Cyclin D1 (CCND1).Conclusions Radiation down-regulated the level of miR-203 in thymic,which promoted radiation-induced thymic lymphoma by targeting TBK1,SNAI2 and CCND1.
