The mechanism of non-thermal effect of 2.45 GHz microwave on HeLa cell apoptosis
10.3760/cma.j.issn.0254-5098.2014.12.007
- VernacularTitle:2.45 GHz微波非热效应诱导人宫颈癌细胞凋亡及其机制
- Author:
Yang YU
;
Huiling CAO
;
Ye XU
;
Shibing LIU
- Publication Type:Journal Article
- Keywords:
Microwave;
Non-thermal effect;
HeLa cells;
Cell apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(12):908-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 2.45 GHz microwave on the apoptosis of HeLa cells and explore the underlying mechanism.Methods HeLa cells were divided into five groups randomly:control group,sham-irradiation group,and three irradiation groups exposed with 10,15 and 20 mW/cm2 2.45 GHz microwave for 10 min.Cell survival was tested by MTT assay,cell nucleus fashion was observed by a laser scanning confocal microscope,cell cycle distribution was measured by flow cytometry,and cell protein expressions were detected by Western blot.Results The cell survival in control group,shamirradiation group and low dose irradiation group had no difference but it became lower in the middle and high dose irradiated group(t =-16.47,-14.23,P ＜ 0.05).The abnormal rates of nuclear fashion in the low,middle and high dose irradiation groups were obviously higher compared with control group (t =9.37,11.25,8.47,P ＜ 0.05),while high dose irradiation group was higher than the low and middle dose group (t =15.32,10.25,P ＜0.05).The rate of HeLa cells in sub-G1 in the irradiation groups were higher than that in control group (t =15.24,22.31,10.69,P ＜ 0.05).The results of immunohistochemistry showed that the expression of Bax and Caspase-3 in irradiation groups was significantly increased,and the expression of Bcl-2 was decreased.Conclusions 2.45 GHz microwave can induce HeLa cells apoptosis with a positive correlation with microwave dose.
