Study of radiosensitization of chloroquine on esophageal cancer cell line
10.3760/cma.j.issn.0254-5098.2014.11.006
- VernacularTitle:氯喹对食管癌细胞系放射增敏的实验研究
- Author:
Xiaoli YUAN
;
Tao LI
;
Jianming HUANG
;
Xiao ZHA
;
Bifang DENG
;
Jinyi LANG
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Autophagy;
Chloroquine;
Radiosensitivity
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(11):823-826
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility of chloroquine radiosensitization of esophageal cancer cell line TE-1 and its further mechanism.Methods Effect of chloroquine on cell viability of TE-1 cells was determined by MTT method.Expression of LC3,Beclin-1 and formation of acidic vesicular organelles (AVOs) were determined by Western blot,and fluorescence staining with Lyso-Tracker Red DND-99,respectively.Clonogenic survival of TE-1 cells was examined by clonogenic forming assay.Results Chloroquine showed dose-dependent inhibition of TE-1 cell growth,and its values of IC50 and IC10 were (72.33 ± 5.28) and (15.42 ± 3.33) μmol/L,respectively.The expression of Beclin-1 and LC3-Ⅱ/Ⅰ markedly increased in irradiated TE-1 cells.The addition of chloroquine with IC10concentration significantly reduced the fluorescence and intensity of AVOs accumulation in the cytoplasm of TE-1 cells.Clonogenic survival fraction decreased obviously,in TE-1 cells with addition of chloroquine after radiation and the value of SERD0 was 1.439.Conclusions Chloroquine could radiosensitize esophageal cancer cells by blocking autophagy-lysosomal pathway and be used as a potential radiosensitizing strategy.
