Effect of allicin on the radiosensitivity of human pancreatic carcinoma BXPC3 cells
10.3760/cma.j.issn.0254-5098.2014.10.006
- VernacularTitle:大蒜素提高人胰腺癌BXPC3细胞放射敏感性的机制研究
- Author:
Hongbing MA
;
Zhengli DI
;
Na HE
;
Jiao WEN
;
Yue KE
- Publication Type:Journal Article
- Keywords:
Allicin;
Bax;
Bcl-2;
Radiosensitivity;
Pancreatic carcinoma;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(10):743-747
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of allicin on the growth and radiosensitivity of human pancreatic carcinoma BXPC3 cells.Methods BXPC3 cells were exposed to X-rays in the presence or absence of allicin.Cell proliferation was measured by MTT assay.Cell cycle distribution and apoptosis were detected by flow cytometry assay.Cell radiosensitivity and the influence of allicin on it was evaluated by colony formation assay.The expressions of Bax and Bcl-2 proteins were assayed by RT-PCR and Western blot.Results IC50 values of allicin on cell growth were 76.24,58.34 and 43.58 μmol/L under 12,24 and 48 h treatment,respectively.Treatment of cells with allicin obviously inhibited cell growth after irradiation and hence increased radiosensitivity (t =2.74,P ＜ 0.05).This treament also enhanced radiation-induced cell cycle arrest at G2/M phase (t =11.41,P ＜0.05),apoptosis induction (t =12.36,P ＜ 0.05),and Bax expression (t =4.83,P ＜ 0.05),but it decreased Bcl-2 expression (t =3.69,P ＜ 0.05).Conclusions Allicin could inhibit cell growth,induce cell cycle arrest and apoptosis via Bax/Bcl-2 pathway and hence increases radiosensitivity of BXPC3 cells.
