Effects of CDT1 gene over-expression on cell apoptosis and cell cycle in irradiation-induced genomic instable liver cells

VernacularTitle:CDT1基因过表达对辐射诱发基因组不稳定肝细胞HL-7702凋亡及细胞周期的影响
Author: Yayi YUAN ; Xuhong DANG ; Yahui ZUO ; Hongyan LIU ; Ruifeng ZHANG ; Zhongxin ZHANG ; Yue REN
Publication Type:Journal Article
Keywords: CDT1; Genomic instability; Over-expression; Cell cycle; Cell apoptosis
From: Chinese Journal of Radiological Medicine and Protection 2015;35(2):103-106
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of CDT1 gene over-expression on the apoptosis and cell cycle distribution in liver cells with a characteristic of genomic instability induced by radiation(GIR).Methods Lentivirus particles were transferred into liver cells of GIR to up-regulate the expression of CDT1 gene.The apoptosis and the cell cycle were detected by flow cytometry (FCM).The expression changes of p53,ATM,ATR,Bcl-2,and Caspase-3 genes were analyzed by real-time fluorescence quantitative PCR.Results CDT1 gene was efficiently increased by the gene transfection(t =15.56,P ＜ 0.05).In the CDT1 over-expressed cells,while the apoptosis ratio was increased (t =4.19,P ＜ 0.05),the expressions of p53 and Bcl-2 gene were decreased (t =-4.21,-2.06,P ＜ 0.05),but the expression of ATM,ATR and Caspase-3 changed with no significant difference compared with control.Conclusions Over-expression of CDT1 could regulate genomic instability through apoptosis pathway and checkpoint independent of p53.