Effect and mechanism of neuroepithelial cell transforming gene 1 on cellular radiosensitivity

VernacularTitle:神经上皮细胞转化基因1对细胞辐射敏感性的影响及其机制探讨
Author: Chang XU ; Yan WANG ; Liqing DU ; Qin WANG ; Qiang LIU
Publication Type:Journal Article
Keywords: Net1; Radiosensitivity; DNA double-strand break; Non-homologous end joining
From: Chinese Journal of Radiological Medicine and Protection 2015;35(1):49-52
CountryChina
Language:Chinese
Abstract: Objective To study the effect of neuroepithelial cell transforming gene 1 (Net1) on the cellular radiosensitivity and underlying mechanism.Methods Real-time quantitative PCR was used to measure the variations in Net1 expression level upon irradiation.Radiosensitivity was analyzed by colonyforming assay after Net1-siRNAs.Net1-associated proteins were identified by co-immunoprecipitation.Results The Net1 mRNA level in the cells was increased significantly (t =-10.52,P ＜ 0.05) after irradiation.Compared to the control group,siRNA-mediated silencing of Net1 enhanced cell radiosensitivity (t =15.31,11.65,P ＜0.05).Net1 was found to interact with Ku70,Ku80 and DNA-PKcs under either normal conditions or after irradiation.Conclusions Net1 could protect cells from irradiation by interaction with DNA repair proteins in non-homologous end joining pathway.