The level of miR-206 in per ipheral blood mononuclear cells and their signicance in systemic lupus erythematosus patients
10.3760/cma.j.issn.1007-7480.2015.04.009
- VernacularTitle:微小RNA-206在系统性红斑狼疮患者外周血单个核细胞中表达的变化
- Author:
Qinglei XU
;
Baolin ZHU
;
Xiaobo MA
;
Wei ZHANG
;
Lingling LIU
;
Shengjun WANG
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
MicroRNAs;
Kruppel-like factor 4;
RORγt
- From:
Chinese Journal of Rheumatology
2015;19(4):254-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of miR-206 in peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients.Methods Human PBMCs were isolated by standard densitygradient centrifugation over Ficoll-Hypaque solution in SLE and healthy controls.Total RNAs were extracted from PBMCs which were stimulated by PMA and ionomycin,thenthe RNA was transcribed reversely into cDNA.The expression of miR-206 and Kruppel-like factor 4 (KLF4) and the orphan nuclear receptor RORγt mRNA was detected by real-time quantitative polymerase chain reaction (qRT-PCR) method.T test and Spearman's correlation test were used for statistical analysis.Results The expression of miR-206 in the PBMCs of SLE patients was significantly decreased compared with that of healthy controls (0.066±0.021 vs 0.143±0.059,t=3.136,P＜0.01).The levels of KLF4 and RORγt mRNAin the PBMCs of SLE patients were increased significantly than those of healthy controls (0.637 ±0.186 vs 0.104 ± 0.028,t=6.673,P＜0.01),(0.575±0.263 vs 0.065±0.014,t=7.386,P＜0.01).Furthermore,there was a negative correlation between the expression of miR-206 and KLF4 or RORγt mRNA in SLE patients (r=-0.627,P＜ 0.01),(r=-0.853,P＜0.01).Conclusion These results indicate that the augmented expression of KLF4 mRNA may be caused by the attenuated expression of miR-206,and the high level of KLF4 mRNA evokes the proportion of Thl7 cells in SLE patients.
