Characters of bone marrow mesenchymal stem cells of primary biliary cirrhosis
10.3760/cma.j.issn.1007-7480.2014.11.002
- VernacularTitle:原发性胆汁性肝硬化骨髓间充质干细胞的功能研究
- Author:
Li WANG
;
Qin HAN
;
Fang KONG
;
Hua CHEN
;
Yongzhe LI
;
Fengchun ZHANG
;
Chunhua ZHAO
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis,biliary;
Mesenchymal stem cells;
Cytokines
- From:
Chinese Journal of Rheumatology
2014;18(11):726-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth and exocrine function of BM-MSC derived from PBC patients.Methods To compare the growth patterns and cytokines secretions between PBC patients and healthy controls by student's t test.Results ① There was no difference in growth profile and speed between PBC patients and healthy controls.② The level of TGF-β1 was much lower in the supernatant of BM-MS from OBC patients than health controls [(2.6±1.9)vs (8.2±6.7)ng/ml,t=-3.641,P=0.001].There were no other differences between two groups' BM-MSC.③ The super natant concentration of interlukin-10 of the third BM-MSC subculture from healthy controls was lower than that of the primary subculture [(18.5±5.0) vs (12.4±3.1) pg/ml,t=2.368,P=0.045],and that of hepatic growth factor from the second subcuhure was higher than the primary subculture [(0.21±0.07) vs (0.35±0.08) ng/ml,t=-2.874,P=0.021].There were seldom discrepancies in other cytokines between different generations of BM-MSC.Conclusion BM-MSC from PBC patients may have almost the similar characters in growth pattern and cytokines secretion as,except the TGF-β1,which was much lower than those from healthy controls.The second subculture of BM-MSC might be more suitable for the treatment to patients with PBC.
