Early assessment of left ventricular dysfunction in mice with doxorubicin-induced cardiotoxicity by high-frequency echocardiography
10.3760/cma.j.issn.1004-4477.2014.12.017
- VernacularTitle:高频超声心动图评价阿霉素对小鼠心功能的早期影响
- Author:
Yuhui ZHANG
;
Lianghua XIA
;
Jiamei JIN
;
Bo ZHANG
;
Ming CHEN
- Publication Type:Journal Article
- Keywords:
Echocardiography;
Doxorubicin;
Ventricular function,left;
Mice
- From:
Chinese Journal of Ultrasonography
2014;23(12):1070-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of high frequency echocardiography on mice with doxorubicin-induced cardiotoxicity and find out the most valuable monitoring indexes for early doxorubicin-induced myocardial damage.Methods Forty mice were divided into doxorubicin-injection group (ADM group) and control group randomly.Twenty mice in each group were injected with doxorubicin and saline at the same dose for 5 weeks continuously.The parameters of cardic function of ADM group and contol group were observed by high-frequency echocardiography after infection.Brain natriuretic peptide (BNP) tested by euzymelinked immunosorbent assay(ELISA) and pathological findings were used as the golden standard.Results ①The left ventricular long axis view and the apical four chamber view could be displayed clearly by transthoracic high-frequency echocardiography.②After 5 weeks intervention,weight,heart rate,left ventricular ejection fraction (LVEF),left ventricular fractional shortening (LVFS),E/A,E wave deceleration time(EDT) and other parameters showed marked difference between ADM group and control group(P ＜0.05).③The decrease of diastolic function was earlier than systolic function in ADM group.④LVEF and E/A presented good negative linear correlation to BNP(r =-0.622,P ＜0.05; r =-0.593,P ＜0.01).EDT showed positive linear correlation to BNP(r =0.501,P ＜0.05).Conclusions Cardic function of mice with doxorubicin-induced myocardial damage could be early evaluated accurately by high-frequency echocardiography,which contained good relativity with BNP.
