Study of low-frequency ultrasound suppress invasion of human prostate cancer cell PC-3
10.3760/cma.j.issn.1004-4477.2014.09.022
- VernacularTitle:低频超声抑制人前列腺癌细胞PC-3侵袭的研究
- Author:
Wenkun BAI
;
Yu WANG
;
Yini CHEN
;
Wei ZHANG
;
Bing HU
- Publication Type:Journal Article
- Keywords:
Sonication;
Microbubbles;
Prostate neoplasms;
Neoplasm invasiveness
- From:
Chinese Journal of Ultrasonography
2014;23(9):807-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether ultrasound combined with microbubbles induces suppress invasion in androgen-independent prostate cancer cells PC-3 and to identify the probable mechanism.Methods Ultrasound with a frequency of 21 kHz and intensity of 46 mW/cm2 in continuous wave mode was used.Ultrasound combined with microbubbles (200 μl SonoVue) was used to treat androgen-independent human prostate cancer PC-3 cells for 30 seconds.PC-3 cells were divided into three groups:control group,ultrasound group and ultrasound combined with microbubbles group.Twelve hours after treatment,cell growth curve we drawn,and transwell chamber model was used to do cell invasion experiments in vitro.Real-time PCR was used to measure the expression matrix metalloproteinase-2 and matrix metalloproteinase-9 messenger ribonucleic acid.Results Twelve hours after treatment,cell growth was not significant difference among three groups (P ＞ 0.05).Twelve hours after treatment,ultrasound combined with microbubbles could significantly inhibit the invasion of human prostate cancer cells (P ＜0.05).Treatment with ultrasound combined with microbubbles down-regulated the expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 messenger ribonucleic acid.Conclusions Ultrasound combined with microbubbles inhibited the invasion in human androgen-independent prostate cancer cells through down-regulation of matrix metalloproteinase-2 and matrix metalloproteinase-9.
