Primary Schwann cell growth on electrospun polymethyl methacrylate nanofiber scaffolds
10.3760/cma.j.issn.1001-8050.2015.01.019
- VernacularTitle:原代大鼠雪旺细胞在聚甲基丙烯酸甲酯电纺纳米纤维支架上的生长
- Author:
Haijian XIA
;
Dan LIU
;
Dong ZHONG
;
Yi YAN
;
Yongzhi XIA
;
Wenyuan TANG
;
Xiaochuan SUN
- Publication Type:Journal Article
- Keywords:
Tissue scaffolds;
Polymethacrylic acids;
Schwann cells
- From:
Chinese Journal of Trauma
2015;31(1):75-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of electrospun polymethyl methacrylate (PMMA) nanofibers with different topological structures as scaffolds for growth of Schwann cells (SCs).Methods Electrospun PMMA nanofibers with random or aligned topological structures were fabricated and measured with biocompatibility.Lentivirus-transfected green fluorescent protein was used as the reporting gene to monitor form and growth manner of SCs on different substrates and dependency of cell body and process with fiber structure,with PMMA thin films served as the control.Results Electrospun PMMA nanofibers revealed good biocompatibility and could exert contact guidance to the growth of SCs.Topological structures of the electrospun nanofibers influenced cell morphology.SCs were aligned with the orientation of substrate fibers and form longer cell process when growing on aligned nanofibers (P ＜0.01).Primary SCs preferred to follow the cue of aligned nanofibers compared to random fibers.Conclusion Aligned electrospun PMMA nanofibers have the potentiality as transplantable scaffolds for loading SCs after neural injury.
