Effects of VEGF/bFGF sustained release microspheres combined with hyperbaric oxygen therapy on survival of uhralong random pattern flaps in rats
10.3760/cma.j.issn.1008-6315.2014.11.010
- VernacularTitle:血管内皮生长因子和碱性成纤维细胞生长因子缓释微球联合高压氧对超长随意皮瓣成活的影响
- Author:
Xueguan XIE
;
Mei ZHANG
;
Mingsheng DING
;
Yankun DAI
;
Shengdong MENG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor;
Basic fibroblast growth factor;
Microspheres;
Hyperbaric oxygen therapy;
Uhralong random pattern flaps
- From:
Clinical Medicine of China
2014;30(11):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of VEGF/bFGF sustained release microspheres combined with hyperbaric oxygen therapy on survival rates of uhralong random pattern flaps in rats.Methods Modified McFarlane flap rat models were randomly divided into 4 groups,VEGF/bFGF microspheres + hyperbaric oxygen group,VEGF/bFGF microspheres group,hyperbaric oxygen group and control group.At the end of 7 days,survival rates of flaps were calculated (Survival rate =Survival area/total area × 100％).The tissue samples were taken from middle portion of flaps to detect the neovascularization.Immunohistochemistry was used to detect the differential expression of VEGF.Results Seven days after the random pattern flap operation in rats,the survival rates of VEGF/bFGF microspheres combined with hyperbaric oxygen therapy experimental group,VEGF/bFGF microspheres experimental group,hyperbaric oxygen therapy experimental group,and control group were (89.54 ± 3.23) ％,(73.54 ± 4.57) ％,(71.89 ± 2.26) ％,(50.36 ± 2.37) ％ respectively.The neovascularization counts of each group in part Ⅱ were (35.14 ± 4.21)/mm2,(23.34 ± 2.53)/mm2,(25.22 ±2.73)/mm2 and (17.37 ± 5.73)/mm2 respectively.VEGF cumulative absorbance in VEGF/bFGF microspheres + hyperbaric oxygen group,VEGF/bFGF microspheres group,hyperbaric oxygen group and control group were 78.39 ± 9.12,52.42 ± 13.59,49.84 ± 12.93,29.24 ± 10.35 respectively.In each experimental result group,there were significant difference between the combination group and the other groups (F =189.956,P ＜ 0.05),but there was no significant difference between VEGF/bFGF microspheres experimental group and hyperbaric oxygen therapy experimental group.Conclusion VEGF/bFGF sustained release microspheres combined with hyperbaric oxygen therapy can promote flap neovascularization,increase the flap blood supply and then improve the survival of random pattern skin flaps.
