Effects and mechanism of different doses of rosuvastatin on expression of liver X receptor and caveolin-1 in cultured human monocyte-macrophage cells induced by oxidized low density lipoprotein
10.3760/cma.j.issn.1008-6315.2014.11.006
- VernacularTitle:不同剂量瑞舒伐他汀对氧化修饰低密度脂蛋白诱导单核-巨噬细胞的肝X受体及小凹蛋白1表达的影响
- Author:
Zheng WEI
;
Yongxin WU
;
Lina LIU
;
Pengjun JI
;
Xiaoyu SHEN
- Publication Type:Journal Article
- Keywords:
Rosuvastatin;
Oxidized low density lipoprotein;
Liver X receptor;
Caveolin-1;
Reverse cholesterol transport
- From:
Clinical Medicine of China
2014;30(11):1138-1141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different doses of rosuvastatin on expression of liver X receptor(LXR) and caveolin-1 in cultured human monocyte-macrophage cells which induced by oxidized low density lipoprotein (ox-LDL).Methods The human monocyte-macrophage cells were divided into six groups:control group,ox-LDL group,different doses of rosuvastatin group (0.01 μmol/L,0.1 μmol/L,1 μmol/L,5 μmol/L).The expression of LXR mRNA and caveolin-1 mRNA were assayed by RT-PCR.Results LXR mRNA expression induced by ox-LDL in the control group and ox-LDL group were 1.00 ± 0.02,0.26 ± 0.02,and the difference was significant (t =56.39,P ＜ 0.001).Meanwhile,caveolin-1 mRNA expression in ox-LDL is (0.27 ± 0.01) fold than that in control (t =31.27,P ＜ 0.001).Meanwhile,There were significant differences among ox-LDL group and the different doses of rosuvastatin group in terms of LXR mRNA and caveolin-1 mRNA expressions (F =72.154,66.007,P ＜ 0.001).Along with the increase the doses of rosuvastatin,there was an increased trends of LXR mRNA and caveolin-1 mRNA expressions (P ＜ 0.05).Conclusion Rosuvastatin and upregulated the LXR mRNA and caveolin-1 mRNA expressions in a dose dependent manner.
