Effects of different doses of 60Co γ-ray irradiation on apoptosis of rat submandibular gland cells
10.3760/cma.j.issn.1008-1372.2014.12.013
- VernacularTitle:不同剂量60 Co γ射线照射对大鼠颌下腺凋亡的影响
- Author:
Fugui WEI
;
Wenzhong SUN
- Publication Type:Journal Article
- Keywords:
Gamma rays/therapeutic use;
Radiation injuries;
Submandibular gland;
Apoptosis
- From:
Journal of Chinese Physician
2014;16(12):1630-1633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effects of different doses of 60Co γ-ray irradiation on apoptosis of rat submandibular gland dells.Methods Wistar rats were randomly divided into four different radiotherapy dosage groups (0 G y,7.5 G y,15 Gy,and 22.5 Gy).Rats in each radiotherapy groups were irradiated by the required dose of γ-ray irradiation (0 Gy,7.5 Gy,15 Gy,22.5 Gy) at one time.Immunohistochemistry method and TdT-mediated dUTP nick end labeling (TUNEL) methods were used to detect expression of P53,caspase-3,and apoptosis of submandibular gland dells in rats.Results The expression levels of P53 and caspase-3 of rat submandibular cells were significantly increased in different radiotherapy dosage groups (7.5 G y,15 Gy,and 22.5 Gy) relative the 0 Gy dosage group.The apoptotic cells were more commonly seen in duct cells in different radiotherapy dosage groups,and the apoptotic cells were gradually increased with the increased radiotherapy dosages.Conclusions 60 Co γ-ray irradiation could lead to apoptosis of rat submandibular gland in the early stage.There is a dose-effect relationship between 60Co γ-ray irradiation and induced apoptosis of rat submandibular gland cells.
