Effect of ABCE1 gene silencing by electroporation on proliferation and migration of human glioma cells line U87MG
10.3760/cma.j.issn.1008-1372.2014.11.016
- VernacularTitle:电转法沉默ABCE1基因对人脑胶质瘤U87MG细胞增殖和迁移能力的影响
- Author:
Tianke WANG
;
Jue HE
- Publication Type:Journal Article
- Keywords:
Gene silencing;
Brain neoplasms/pathology;
Glioma/pathology;
ATP-binding cassette transporters/genetics;
Tumor cells,cultured;
Transfection;
Cell proliferation;
Cell movement
- From:
Journal of Chinese Physician
2014;16(11):1508-1512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ATP-binding cassette protein E 1 (ABCE1) gene silencing by electroporation on the survival,cell cycle and invasion of human glioma cells line U87MG.Methods The siRNA against ABCE1 was constructed and transfected into U87MG cells by electroporation.The expression of ABCE1 was detected by real time-polymerase chain reaction (RT-PCR) and Western blot.Flow cytometry was used to detect the cell cycle distribution and apoptosis.The effects of ABCE1 gene silencing by electroporation on proliferation,migration and invasion of U87MG cell line were evaluated by cell counting kit-8 (CCK-8) assay,wound closure assay,chemotactic migration,and cell invasive experiments,respectively.Results Compared to the control and blank groups,the mRNA and protein levels were significantly decreased in the experimental group when ABCE1 gene silencing by electroporation.The cell cycle was arrested at G0/G1 phase,and cell number in S phase was decreased in U87MG cell line (P ＜ 0.05).The cell growth inhibition ratio in the experimental group was significantly higher than that in the control and blank groups (P ＜0.01).Compared to the control and blank groups,the experimental group U87MG cell proliferation was inhibited significantly (P ＜ 0.05).Scratch healing experiments showed the experimental group migration ability was decreased significantly (P ＜ 0.05).Transwell chamber method showed the experimental group U87MG cell invasion ability was decreased significantly (P ＜ 0.05).Conclusions ABCE1 is involved in the progression of human glioma cells,and inhibiting the expression of ABCE1 by electroporation can decrease migration,invasion,and proliferation ability of tumor cells in vitro.
