Inhibitory effects of lobaplatin on human ovarian cancer and its regulation of apoptosis-related genes in nude mice
10.3760/cma.j.issn.1008-1372.2015.01.023
- VernacularTitle:洛铂对卵巢癌裸鼠移植瘤的抑制作用和对凋亡相关基因调控的研究
- Author:
Dongmin WEI
;
Hongyan YU
;
Hui WANG
;
Wenli LIU
;
Sufang SHI
- Publication Type:Journal Article
- Keywords:
Organoplatinum compounds/pharmacology;
Disease models,animal;
Ovarian neoplasms/drug therapy;
Mice,nude;
Neoplasm transplantation;
Genes,bcl-2;
Gene expression;
Apoptosis
- From:
Journal of Chinese Physician
2015;17(1):81-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effects of lobaplatin and cisplatin and their regulation of apoptosis-related genes in ovarian cancer cells in nude mice.Methods SKOV3 cells were implanted into nude mice.In monotherapy treatment study,the nude mice bearing human SKOV3 cells were randomly divided into control,lobaplatin,and cisplatin groups,with 7 mice in each group.The mice in each group were received corresponding treatment.The volume of tumor and the weight of nude mice were measured three times per week,respectively.Tumor inhibitory rate was calculated.The protein expressions of bax and bcl-2 were detected by flow cytometry.Results The growth inhibitory rate was 47.2％ in lobaplatin group and 42.8％ in cisplatin group,without significant difference between two groups (P ＞ 0.05).The expression of bcl-2 was decreased but the bax was increased in lobaplatin and ciaplatin groups compared to the control group.Conclusions Lobaplatin can significantly inhibit the growth of ovarian cancer cells,induce apoptosis by down-regulation of bcl-2 and up-regulation of bax.
