Influence of brain-derived neutrophic factor on NF-κB, TNF-α, and cell apoptosis in rats with cerebral ischemia-reperfusion injury
10.3760/cma.j.issn.1008-1372.2014.12.017
- VernacularTitle:脑源性神经营养因子对脑缺血再灌注损伤大鼠核转录因子、肿瘤坏死因子-α和细胞凋亡的影响
- Author:
Weijun YU
;
Zhi YU
;
Boda LU
;
Kaide LIU
;
Laiming FANG
- Publication Type:Journal Article
- Keywords:
Brain-derived neurotrophic factor;
Brain ischemia;
Reperfusion injury;
NF-kappa B;
Tumor necrosis factor-alpha;
Apoptosis
- From:
Journal of Chinese Physician
2014;16(12):1645-1648
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of nuclear factor-κB (NF-κB),tumor necrosis factor-α(TNF-α),and cell apoptosis of cerebral ischemia-reperfusion injury and the influence of brain-derived neutrophic factor(BDNF) on these parameters in rats.Methods Eighty-four male Sprague-Dawley (SD) rats were randomly divided into two groups:BDNF (n =42) and control (n =42) groups.The BDNF group was induced using the improved Zea-longa method and were received abdominal injections of BDNF (0.5 μg/μl) immediately after injury.The control group was received abdominal injections with the same dose sodium chloride injection immediately after injury and repeated one time everyday until the rats was killed.Each group was divided into seven subgroups by sacrificed time after injury,including subgroups 1 h,3 h,6 h,12 h,24 h,3 d,and 7 d; each subgroup got 6 rats.Each subgroup were randomly selected three rats after being killed.The expressions of NF-κB and TNF-α of rats contusion peri tissues brain tissue were detected by immunohistochemical methods.Terminal deoxynucleotidyl transferase (TUNEL) method was used to observe the peri cell apoptosis after brain contusion.Results The expressions of NF-κB and TNF-α in BDNF group was significantly decreased relative to the control group (P ＜ 0.05),with a significant positive correlation between two parameters in two groups (P ＜ 0.001).The number of apoptotic cells was significantly decreased in the BDNF group relative to control group (P ＜ 0.05).Conclusions Brain-derived neutrophic factor probably relieves inflammation response,reduces the change of secondary brain injury after traumatic brain injury,and decreases neural cell apoptosis,and finally provides protection of neurocytes.
