Tea polyphenols prevents renal fibrosis of diabetic nephropathy via inhibiting TGF-β signaling pathway
10.3760/cma.j.issn.1008-1372.2014.10.005
- VernacularTitle:茶多酚通过抑制TGF-β信号通路缓解小鼠糖尿病肾病肾纤维化进程
- Author:
Li CHEN
;
Jing LI
;
Jiaoe ZENG
;
Shangxia NING
- Publication Type:Journal Article
- Keywords:
Tea;
Phenols/pharmacology;
Transforming growth factor beta;
Diabetic nephropathies;
Fibrosis;
Disease models,animal;
Mice
- From:
Journal of Chinese Physician
2014;16(10):1322-1327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and its mechanisms of tea polyphenols (TP) in the prevention of diabetic renal fibrosis.Methods Mouse model of diabetes mellitus was induced by high-fat diet combined with intraperitoneal injection of streptozotocin (STZ,50 mg/kg).Real-time quantitative polymerase chain reaction (RT-PCR) and Western blotting were used to determine the transcription and expression of genes related to the renal fibrosis.Results Diabetic mouse model was successfully established by injection of high-fat diet plus STZ.The transcription of E-cadherin was decreased and transcriptions of genes related to the renal fibrosis such as transforming growth factor β1 (TGF-β1),vimentin,α-smooth muscle actin (α-SMA),fibronectin,collagen Ⅰ,and collagen Ⅳ were increased in the renal tissues of diabetic mice,which indicated the diabetic nephropathy with renal fibrosis in diabetic mice.Treatment of diabetic mice with TP for 8 weeks could reverse the process of diabetic nephropathy with renal fibrosis in accompanied with the increase of E-cadherin transcription and decrease of TGF-β1-targeted genes such as vimentin,α-SMA,fibronectin,collagen Ⅰ,and collagen Ⅳ in the renal tissues of diabetic mice.Moreover,incubation of normal rat kidney proximal tubular epithelial cell line (NRK-52E) cells with TGF-β1 could also induce the changes in fibrotic morphological and transcription or expression of fibrosis related genes,and all effects of TGF-β1 could be inhibited by TP treatment in NRK-52E cells.Conclusions These results indicate that chronic treatment with TP may relieve renal fibrosis of diabetic nephropathy through the inhibition of TGF-β signaling pathway and provide important experimental data for the prevention and treatment of diabetic nephropathy.
