Process study of brain protein hydrolysate of inactivate and virus removal
10.3760/cma.j.issn.1008-6706.2015.01.018
- VernacularTitle:脑蛋白水解物溶液的灭活/去除病毒工艺研究
- Author:
Fengxia LI
;
Yunhai CHU
;
Qingling HUANG
;
Hongwei LI
- Publication Type:Journal Article
- Keywords:
Protein hydrolysate;
Brain;
Technology,pharmaceutical;
Parvovirus,porine;
Herpesvirus2,bovine
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;22(1):54-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the process of brain protein hydrolysate of inactivate and virus removal.Methods The Parvoviridae parvovirus genera of porcine parvovirus (PPV),vesicular stomatitis virus rhabdovirus genera of vesicular stomatitis virus (VSV) were chosen as a model virus,wherein PPV represents no envelope deoxyribonucleic acid(DNA) virus,VSV represents the envelope ribonucleic acid(RNA) virus.Simulation of the production process of virus inactivation steps 100 ℃ × 30 min,ultrafiltration as inactivation/removal condition.The virus respectively according to 1 ∶ 9 into the brain protein hydrolysate,high temperature and ultra filtration virus inactivation/removal.In pig kidney cells (PK-15) in PPV cell culture,Africa green monkey kidney cells(Vero cells) cultured VSV,determination of virus titer.Results PPV and VSV through the sterilization,virus median tissue culture infective dose(TCID50) were 6.15log/0.1mL(logs),5.37 log/0.1mL(logs) ;removal processaverage virus reduction coefficient were 6.15log/0.1mL(logs),5.37 log/0.1mL(logs).Conclusion The high temperature and ultra filtration produces brain protein hydrolysate solution process are effective virus inactivation/removal process.
