Effects of simvastatin on neurons autophagy in hippocampus following intrac-erebral hemorrhage in rats

VernacularTitle:辛伐他汀对实验性脑出血大鼠海马神经细胞自噬的影响
Author: Yingzi LIU ; Honghu WU ; Jiyan ZHANG
Publication Type:Journal Article
Keywords: Simvastatin; ICH; Brain edema; Nerve function; Autophagy
From: Chinese Journal of Immunology 2015;(7):902-905
CountryChina
Language:Chinese
Abstract: Objective:To evaluate effect of simvastatin on neurons autophagy in the hippocampus following intracerebral hemorrhage( ICH) in rats.Methods:All rats were divided randomly into sham group;ICH group and SIM group.The rat model of ICH was made by injection of Ⅶ type collagenase.The behavioral changes were evaluated with Garcia′s neurologic deficit score.Wet-dry weight method was used to evaluate brain edema.Additionally,localization of LC3 protein was determined by immunohistochemistry, and the expression of Beclin-1 in hippocampus was tested by Western blot methods.Results: Compared with ICH group,simvastatin treatment after ICH significantly improved neurologic deficit score ( P<0.05 ) , reduced the water content of brain tissue ( P<0.05 ) , inhibited the expression of LC3 and Beclin-1 protein(P<0.05).Conclusion:Our findings indicated that administration of simvastatin had neuroprotective effects in ICH rat model,which could be involved in inhibiting activation of autophagy in the hippocampus,finally reducing brain edema and neurobehavioral deficit.