Genotyping analysis of epidemic strains ofMycoplasma pneumoniae in Suzhou area

VernacularTitle:肺炎支原体流行株基因分型研究
Author: Dong LI ; Liping FAN ; Huiquan SUN ; Chuangli HAO ; Xuejun SHAO ; Jun XU
Publication Type:Journal Article
Keywords: Mycoplasma pneumonia; P1 gene; genotyping
From: Journal of Clinical Pediatrics 2015;(4):338-341
CountryChina
Language:Chinese
Abstract: ObjectiveTo analyze the genotype and variation ofMycoplasma pneumonia (MP) strains isolated from chil-dren with MP infection in Soochow area.MethodsThe nasopharyngeal secretions from hospitalized children with MP infection were collected during January 2012 and December 2013. The nested-multiplex PCR based on MPP1 gene was performed to detect the subtype ofMP gene.ResultsIn 313 samples, 304 (97.12 %) samples were classiifed as P1-I type and 8 (2.56%) sam-ples were classiifed as P1-II type and one (0.32%) was V2 variant. Gene sequencing results were consistent with nested-multiple PCR results.ConclusionsNested-multiplex PCR is a reliable method for genotyping of MPP1 gene. During the study period, P1-I type was the common genotype and only one case of V2 variant was found.