ClassⅠ integron and its correlation with genes coding forESBLs in ESBLs-producing Escherichia coli from chil-dren
10.3969/j.issn.1000-3606.2015.04.014
- VernacularTitle:儿童产超广谱β-内酰胺酶大肠埃希菌Ⅰ类整合子及其与ESBLs基因关系的研究
- Author:
Hongchao JIANG
;
Liyue KUI
;
Hailin HUANG
;
Min SU
;
Mao FAN
- Publication Type:Journal Article
- Keywords:
integron;
drug resistant gene;
extended-spectrum β-lactamases;
child
- From:
Journal of Clinical Pediatrics
2015;(4):345-347
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the correlation with Genes Coding forESBLs and ClassⅠIntegron in ESBLs-producing Escherichia coli from children.MethodsPCR was used for gene typing detection of 100 strains of ESBLs-producingEsche-richia colistrains. While detection of class I integrase gene in the 100 strains ESBLs-producingEscherichia coli and 100 strains of non-ESBLs producingEscherichia coli were separately performed by PCR. It provides the solid base not only to reveal the relationship between class I integron and drug resistance, but also the relationship between class I integron and ESBLs-producing. ResultsThe most frequently genotyping from ESBLs-producingEscherichia coli in children isCTX-M (84%), followed by TEM-1(63%). The predominant distribution of genotype in ESBL- producing strains isTEM-1 +CTX-M (45%), followed by CTX-M (34%). Class I integrase gene detected in ESBLs- producing and non- ESBLs producing strain were 100 cases (100%) and 25 cases (25%) separately, the difference was statistically signiifcant (P<0.05); drug resistance in class I integron positive strains were signiifcantly higher than in class I integron negative strains, especially in Ciprolfoxacin, Levolfoxacin, and Amino-glycoside (86.4%, 88%, and 80%).ConclusionsThe distribution of Class I integron in ESBLs-producingEscherichia coli is signiifcantly higher than that in non-ESBLs-producing strains, It is rational that Class I integron highly correlate with strong drug resistance in ESBLs-producing strains.
