Effect of hepatopoietin Cn on liver stem cells
10.7644/j.issn.1674-9960.2015.06.002
- VernacularTitle:肝细胞生成素Cn促进肝干／祖细胞增殖的功能研究
- Author:
Yongfeng LI
;
Yong LIU
;
Jing CHANG
;
Pengfei LIU
;
Huiying GAO
;
Xu ZHOU
;
Jun LU
;
Chunping CUI
- Publication Type:Journal Article
- Keywords:
hepatic stimulating factor;
hepatopoietin Cn;
liver stem cell;
cell proliferation;
liver regeneration;
interleukin-6;
cell movement;
mice,transgenic
- From:
Military Medical Sciences
2015;(6):413-417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hepatopoietin Cn(HPPCn) on liver stem cells.Methods In this study, WB-F344 cell line was used, and MTT and flow cytometry assay were conducted to determine cell proliferation and apoptosis.Transwell assay was used to test the migration of WB-F344 cells.A 2AAF-partial hepatectomy(PH) mouse model was used to observe the effect of HPPCn on liver stem cell proliferation in vivo.Results HPPCn enhanced WB-F344 cell proliferation and migration and activated the SphK1, Erk and Stat3 signal pathways.The analysis of the 2AAF-PH mouse model showed that oval cells in the experimental group far outnumbered those in control and the regeneration of the liver was improved post PH.Conclusion HPPCn can increase the liver stem cell proliferation and survival while promoting the regenenation of the liver by augmenting oval cell proliferation.
