Morphology and location of mitochondria during mitosis impacted by ROS

VernacularTitle:ROS影响有丝分裂时期细胞内线粒体的形态及分布
Author: Yuanyuan BAI ; Youguo LING ; Yong HU ; Yangbo FU ; Lihong QIU ; Fang YAN ; Quanbin XU ; Cheng CAO
Publication Type:Journal Article
Keywords: reactive oxgen species; mitosis; mitochondria
From: Military Medical Sciences 2015;(6):427-431
CountryChina
Language:Chinese
Abstract: Objective To detect the impact of reactive oxygen species ( ROS) on mitochondrial morphology and distri-bution during mitosis.Methods A viral vector in which the fluorescence gene was specifically under the control of mito-chondrial promoter was constructed and confirmed through DNA sequencing and Western blotting.After transfecting HeLa s3 cell with packaged virus, the HeLa s3-COX4tp-EGFP cell line stably expressing the mitochondrial fluorescence signal was obtained.With immunofluorescent staining, the impact of ROS on the morphology and distribution of mitochondria dur-ing mitosis was inspected.Result The cell line constantly expressing mitochondrial fluorescence signals was successfully constructed.Meanwhile,it was found that H2 O2 treatment could significantly change the morphology and distribution of mi-tochondria during mitosis by confocal microscopy.Conclusion Our study demonstrates that ROS can affect the morphology and distribution of mitochondria during mitosis.This research help study the relationship between the mitochondrial function and the regulation of mitosis in the future.