Effect of HCV receptors’ sequence on virus entry
10.7644/j.issn.1674-9960.2015.06.006
- VernacularTitle:靶细胞表面HCV受体分子排列顺序对病毒入侵影响的研究
- Author:
Qiong KANG
;
Jiang DENG
;
Jun XIAO
;
Yanyu ZHANG
;
Ping MA
;
Bo GAO
;
Fan YAN
;
Xipeng ZHOU
;
Jinbo XU
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
transgenic cells;
HCV receptors
- From:
Military Medical Sciences
2015;(6):432-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of HCV receptors′sequence on virus entry based on the two-dimensional structure and via tandem expression of HCV receptors on mouse hepatocytes.Methods The construced recombinant expression vectors pCDH-hLDLR-hSR-BⅠ-hCD81-GFP, pCDH-hLDLR-hCD81-hSR-BⅠ and pCDH-hCLDN-1-hOCLN-DsRed were cotransfected into 293FT cells with package vectors.The collected recombinant lentivirus expressing hCLDN-1-hOCLN was concentrated and attacked mouse hepatocytes.The transgenic mouse hepatocytes with tandem overexpression of CLDN-1 and OCLN were established after G418-selection.The transduced cells LSCCO/Hepa1-6 and LCSCO/Hepa1-6 were sorted via flow cytometry and puro-G418-selection after recombinant lentivirus expressing hLDLR-hSR-BⅠ-hCD81 and hLDLR-hCD81-hSR-BⅠattacked Hepa1-6 respectively.The infectivity of transduced mouse hepatocytes LSCCO/Hepa1-6 and LCSCO/Hepa1-6 to HCV was analyzed via direct-infection of serum-derived virus.Furthermore, the effect of HCV receptors′sequence on virus entry was studied.Results Both LSCCO/Hepa1-6 and LCSCO/Hepa1-6 enhanced HCV-cell binding.The transduced mouse hepatocytes LSCCO/Hepa1-6 had more HCV endocytosis.Conclusion SR-BⅠhas priority over CD81 in HCV entry in the early stage.
