Punica granatum seed oil inhibits malignant behavior of breast cancer cells
10.7644/j.issn.1674-9960.2015.06.007
- VernacularTitle:石榴籽油抑制乳腺癌细胞恶性生物学行为的研究
- Author:
Guoqiang FU
;
Lu LIU
;
Lei ZHANG
;
Yuan GAO
;
Xiaona XU
;
Feng XIE
;
Feng WANG
- Publication Type:Journal Article
- Keywords:
Punica granatum see d oil;
punicic acid;
breast cancer;
MCF-7;
MDA-MB-231;
proliferation;
apoptosis
- From:
Military Medical Sciences
2015;(6):438-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Punica granatum( pomegranate) see d oil( PSO) on proliferation and apop-tosis behaviors of breast cancer cells.Methods Fatty acid composition was detected by gas chromatography,breast cancer cells, MCF-7 and MDA-MB-231 were treated with PSO, cell proliferation was observed by MMT, cell apoptosis was analyzed by flow cytometry,and expression levels of proliferation and apoptosis-related proteins were detected by Western blot.Results Punicic acid (PA) was the major fatty acid in PSO(74.41%).PSO could inhibit the proliferation while in-ducing apoptosis in both cell lines in a dose-and time-dependent manner, significantly decrease the expression level of Cox-2 and Bcl-2, increase the expression level of Bax and caspase-3 (cleaved),remarkably upregulate the expression of P53 in MCF-7, and downregulate p53 expression in MDA-MB-231.Conclusion PA may be one of the functional ingredients of PSO which can inhibit proliferation and induce apoptosis in breast cancer cells.These effects are probably mediated by regu-lating the expression of Cox-2, Bcl-2, Bax, caspase-3 (cleaved) and p53.
