Protective effect of oxymatrine on vein endothelial cell from glucose toxicity
10.3969/j.issn.1001-1978.2015.04.012
- VernacularTitle:氧化苦参碱对高糖毒性下血管内皮细胞的保护作用
- Author:
Yun YI
;
Qin WU
;
Liping HUANG
;
Shangdong LIANG
;
Yun GAO
- Publication Type:Journal Article
- Keywords:
oxymatrine;
human umbilical vein endo-thelial cells (HUVECs);
high glucose;
cytotoxicity;
A2B receptor;
diabetes
- From:
Chinese Pharmacological Bulletin
2015;(4):499-503
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of oxymatrine (OMT)on protecting human umbilical vein endothelial cells (HUVECs)from toxicity induced by high glucose in vitro.Methods The HUVECs were cultured with medium containing different concentrations of glucose or OMT. The cells were randomly divided into 6 groups:5.5 mmol·L -1 control group(Control),22.2 mmol·L -1 high glucose (22.2 mmol·L -1 ),44.4 mmol·L -1 high glucose (44.4 mmol·L -1 ),control+OMT(control +OMT),22.2 mmol·L -1 high glu-cose +OMT(22.2 mmol·L -1 +OMT),44.4 mmol ·L -1 high glucose +OMT (44.4 mmol · L -1 +OMT).The protective effect of oxymatrine was as-sessed by MTS assays.The expression of A2B in HU-VECs was detected by Real-time quantitative PCR (qPCR)and Western Blot methods.Results The glucose was shown to have caused cytotoxicity in HU-VECs.Oxymatrine (3 μmol·L -1 )was found to have protected HUVECs from glucose toxicity effectively, and reduced the expression of A2B significantly.Con-clusion Oxymatrine can obviously protect HUVECs from cytotoxicity induced by high glucose and the effect is performed partly by decreasing A2B expression.
