Evaluation on detection method of β-lactamase in 3 kinds of Staphylococcus aureus
10.3969/j.issn.1673-4130.2015.07.014
- VernacularTitle:3种金黄色葡萄球菌β-内酰胺酶检测方法的评价
- Author:
Penghao GUO
;
Yali WANG
;
Kang LIAO
;
Jianlong CHEN
- Publication Type:Journal Article
- Keywords:
staphylococcus aureus;
β-lactamase;
method evaluation
- From:
International Journal of Laboratory Medicine
2015;(7):897-898
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the detection methods for β‐lactamase in 3 kinds of Staphylococcus aureus to provide the basis for clinical laboratories selecting the best detection method .Methods 40 strains of Staphylococcus aureus were selected .With the BlaZ gene detection result as the gold standard for β‐lactamase detection ,the detection performance of the penicillin zone‐edge deter‐mination ,cloverleaf assay ,nitrocefin‐disk were evaluated .Results 20 strains of Staphylococcus aureus were the BlaZ gene positive detected by PCR and 20 strains were negative .The sensitivity of the penicillin zone‐edge determination method ,cloverleaf assay and nitrocefin‐disk were all 100 .00% and the specificity were 100 .00% .Conclusion Three kinds of β‐lactamase detection method have better sensitivity and specificity .So different laboratory can choose one of the method to detect β‐lactamase of staphylococcus aureus according to the actual situation .
