Comparability of 5 items of hepatitis B in ELISA by using water bath box and rapid incubation

VernacularTitle:乙肝5项ELISA 水浴箱与快速孵育器使用结果的可比性
Author: Xucai DONG ; Huanhuan SHI ; Fang CHENG ; Man WANG ; Jing XU ; Huizhong ZHANG
Publication Type:Journal Article
Keywords: hepatitis B; water bath; rapid incubation unit
From: International Journal of Laboratory Medicine 2015;(7):923-924
CountryChina
Language:Chinese
Abstract: Objective To investigate and evaluate the comparison results of five items of hepatitis B in ELISA by using water bath box and rapid incubation .Methods 92 patients with hepatitis B in our hospital from June 2012 to June 2013 were randomly se‐lected and 184 cases of healthy physical examination were randomly selected as the controls .The water bath and rapid incubation were adopted to conduct ELISA for simultaneously detecting 5 items of hepatitis B in samples .Results The results of two groups had higher positive coincidence rate .The average OD value of specimens in the water bath was higher .But the specimen S/CO value in the fast average incubation was higher ,its co value was higher than that of the water bath .Conclusion The rapid incubation can be introduced for improving the work efficiency in the daily work ,but the specimen with weak reactiveresult needs to be re‐de‐tected by using the water bath and making records .The fast incubation device is best for only use of the HBsAg reaction ,because the other four reaction process time is shorter ,and there are some non‐conformity results .