Construction and identification Bifidobacterium as a delivery system of IL -10
10.3760/cma.j.issn.1008-6706.2015.08.006
- VernacularTitle:携带IL-10基因双歧杆菌载体的构建及鉴定
- Author:
Dingguo ZHANG
;
Jun YAO
;
Zhongsheng ZHU
;
Mingguang LAI
;
Chen WEI
;
Lisheng WANG
- Publication Type:Journal Article
- Keywords:
Bifidobacterium longium;
IL-10;
Shuttle plasmid;
Construction;
Identification
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(8):1136-1138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and identify a novel IL-10 delivery system by transforming a hIL-10-containing plasmid into B.longum (BL -hIL -10).Methods A plasmid vector pBADs -GFP was selected which had been built by previous test and biosynthetic hIL-10 plasmid,through double enzyme digestion and enzyme reaction,to construct and identify PBADs-hIL-10 shuttle plasmid,then to synthesis BL-hIL-10.hIL-10 was expressed and secreted into the culture supernatant of BL-hIL-10 after 0.2% L-arabinose induction in vitro as examined by Western blot,enzyme-linked immunosorbent assay (ELISA)and RT-PCR;Culture supernatants and bacterium pellets were collected after continuous culture for 12,24 and 36h,respectively.hIL-10 was expressed and secreted into the culture supernatant of BL-hIL-10 after 0.2% L-arabinose induction in vitro as examined by Western blot,enzyme -linked immunosorbent assay (ELISA)and RT -PCR;Culture supernatants and bacterium pellets were collected after continuous culture for 12,24 and 36h,respectively.Results The BL-hIL-10 bacterial strain that can stably express hIL-10 factor was successfully screened out,and the levels of hIL-10 in both superna-tant and cell pellet were similarly reached maximum at 24h of culture.Conclusion BL-hIL-10 as a novel oral hIL-10 delivery system has been successfully established,which established a basis for the treatment of IBS with transgenic Bifidobacterium.
