Expression characteristics of epithelial markers in human embryonic stem cells differentiating into keratinocytes
10.3969/j.issn.1671-167X.2015.02.023
- VernacularTitle:人胚胎干细胞分化为角质形成细胞过程中标志物的表达特点
- Author:
Yulan REN
;
Yuan ZHAN
;
Lu LU
;
Shenglin LI
;
Xin FU
;
Guangyan YU
;
Tong CAO
;
He LIU
- Publication Type:Journal Article
- Keywords:
Embryonic stem cells;
Keratinocytes;
Karyotyping;
Epithelial cells
- From:
Journal of Peking University(Health Sciences)
2015;(2):305-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To differentiate human embryonic stem cells ( hESCs ) into keratinocytes ( K-hESCs) and analyse the expression characteristics of biomarkers of K-hESCs.Methods: The hESCs of line H9 were seeded on matrigel in mTeSR1 medium.The hESCs were directly differentiated into kerati-nocytes in epithelial differentiation medium with bone morphogenetic protein 4, retinoic acid and N2 sup-plement.The karyotype of K-hESCs was analyzed, comparing the gene expression differences of K-hESCs with human gingival epithelial cells (HGECs), human immortalized oral epithelial cells (HIOECs) and HaCaT by Real-time PCR.Molecular characteristics of the cell differentiation were observed throughout the process by immunocytochemical techniques.Results:H9-hESCs were successfully differentiated into the cells that exhibited characteristics of keratinocytes in epithelial differentiation medium.The karyotype of K-hESCs was 46, XX; and the keratinocyte gene p63 expression in K-hESCs was significantly lower than that in HaCaT ( P<0.05) , but there was no significant difference of p63 expression in K-hESCs, comparing with that in HGECs and HIOECs ( P >0.05 ) .Conclusion: H9-hESCs could be directly differentiated into K-hESCs.The gene expression of K-hESCs was similar to that of epithelial cells in the early stage of monolayer cells differentiation with high proliferative activity.
