Osteogenic induction of human bone marrow mesenchymal stem cells cultured in complex medium
10.3969/j.issn.2095-4344.2015.10.005
- VernacularTitle:在复合成分培养基中骨髓间充质干细胞的诱导成骨
- Author:
Lili XU
;
Xiaojuan SUN
;
Xiuxian HAO
;
Tingting XIE
;
Nailong YANG
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cels;
Osteoblasts;
Cel Differentiation
- From:
Chinese Journal of Tissue Engineering Research
2015;(10):1501-1505
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that the number of osteoblasts is often decreased after osteoporosis, and osteoblast replacement therapy becomes a new target for the treatment of osteoporosis. OBJECTIVE:To observe the osteogenic differentiation of human bone marrow mesenchymal stem cels cultured in dexamethasone, vitamin C and beta-glycerophosphate. METHODS:Mesenchymal stem cels were isolated and purified from adult bone marrow using human lymphocyte separation medium. The expression of cel surface markers was detected by flow cytometry. Cel ultrastructure was observed by transmission electron microscope. Then, the bone marrow mesenchymal stem cels were cultured in osteogenic induction medium containing dexamethasone, vitamin C andβ-glycerophosphate, and RT-PCR was used to detect the bone morphogenetic protein-2 mRNA expression after osteogenic induction. RESULTS AND CONCLUSION:A large number of adherent cels were visible as fibrous growth at 2 weeks after culture and strongly expressed CD44, CD29, but did not express CD34, CD45. These cels could be induced to differentiate into osteoblasts, and express bone morphogenetic protein-2 mRNA. Alizarin red staining and alkaline phosphatase staining were positive for the cels. These findings suggest that human bone marrow mesenchymal stem cels cultured in dexamethasone, vitamin C and beta-glycerophosphate can differentiate into osteoblasts, and has a potential for the treatment of osteoporosis.
