Chondrogenic differentiation of mesenchymal stem cells on matrigel/chitosan scaffolds
10.3969/j.issn.2095-4344.2015.10.006
- VernacularTitle:基底膜基质/壳聚糖诱导骨髓间充质干细胞成软骨分化
- Author:
Yun DAI
;
Jing HE
;
Fang WU
- Publication Type:Journal Article
- Keywords:
Bone Marrow;
Mesenchymal Stem Cels;
Basilar Membrane;
Chitosan
- From:
Chinese Journal of Tissue Engineering Research
2015;(10):1506-1510
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies have found that the basement membrane matrix can induced the chondrogenic differentiation of bone marrow mesenchymal stem cels, but there is a wide gap between its mechanical properties and practical application, and further research is needed. OBJECTIVE:To prepare a suitable matrigel/chitosan scaffold that has appropriate mechanical properties and remarkable bioactivity for cartilage repair. METHODS: We selected genipin as cross-linking agent, and mixed Matrigel with cross-linked chitosan at different ratios (2:1, 1:1, 1:3). Then rat bone marrow mesenchymal stem cels were seeded on different scaffolds and cultured for 14 days. The mechanical properties of materials were measured by DMA. Cel counting kit-8, FDA staining, ELISA kits and Alcian blue staining were used to measure the bioactivity of materials. RESULTS AND CONCLUSION:The storage modulus of scaffolds was raised from 0.48 kPa to 1.78 kPa with increase ratio of chitosan. Cels spread wel in the early period on al scaffolds, and then the cels on the chitosan scaffold showed reduced chondrogenic activity, but cels on the scaffolds with matrigel could maintain chondrogenic differentiation. The matrigel/chitosan scaffold at a ratio of 1:1 had appropriate mechanical properties and higher levels of colagen II and colagen X at 14 days. The prepared matrigel/chitosan scaffold with decent mechanical performance can promote the differentiation of bone marrow mesenchymal stem cels into chondrogenic lineages, which can be used in cartilage tissue engineering.
