Effect of miR-375 on viability, cell cycle and apoptosis of colon cancer HCT116 cells
10.3969/j.issn.1000-4718.2015.04.006
- VernacularTitle:miR-375对人结肠癌细胞株 HCT116活性、细胞周期及凋亡的影响
- Author:
Baolong LIU
;
Binwen WU
;
Sujun HUANG
;
Dongfeng LI
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
MicroRNA-375;
Cell viability;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;33(4):609-614
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effect of microRNA-375 ( miR-375) on the viability, cell cycle and apop-tosis of HCT116 cells.METHODS: The expression of miR-375 in different colorectal cancer cell lines was detected by real-time PCR.The miR-375 mimics was transfected into HCT116 cells by LipofectamineTM 2000.The mRNA expression of miR-375 and AEG-1 was detected by real-time PCR.The HCT116 cell viability was detected by MTT assay.The changes of apoptosis and cell cycle distribution were analyzed by flow cytometry.RESULTS:Real-time PCR showed that miR-375 expression was the lowest in HCT116 among 4 colorectal cancer cell lines.The expression level of miR-375 significantly in-creased in miR-375 mimics group compared with that in the negative control group.The high expression level of miR-375 significantly inhibited the mRNA expression of AEG-1.After transfection with miR-375 mimics, the cell viability was in-hibited, the apoptotic rate was increased, the proportion of G1-stage cells was increased, and the proportion of S-stage cells was decreased.CONCLUSION:miR-375 inhibits the viability, mediates the cell cycle arrest and promotes the apoptosis of colon cancer HCT116 cells.miR-375 may act as a tumor suppressor in colorectal cancer by inhibiting AEG-1.
