Energy metabolism and apoptotic effect of microwave radiation on rat myocardial cells
10.3969/j.issn.1000-4718.2015.04.013
- VernacularTitle:微波辐射对大鼠能量代谢及心肌细胞凋亡的影响
- Author:
Wenhe ZHU
;
Nan SHEN
;
Junjie XU
;
Xiuhong ZHONG
- Publication Type:Journal Article
- Keywords:
Microwave radiation;
Energy metabolism;
Apoptosis;
Mitochondria
- From:
Chinese Journal of Pathophysiology
2015;33(4):647-651
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effect of microwave radiation at different intensities on the rat myocardium and its possible mechanism.METHODS:The rats were radiated by the intensity of 500, 1 000, 1 500 and 2 000 W/m2 with 2 450 MHz microwave for 6 min.The heart tissue was collected 6 h after microwave radiation.ATP and mitochondria complexⅣandⅤwere measured.The changes of the tissue structures were observed under transmission electron micro-scope.The apoptosis of the myocardial cells was detected by a cell analyzer.The protein level of cleaved caspase-3 was de-termined by Western blotting.RESULTS:The concentration of ATP and activity of mitochondria complexⅣandⅤsigni-ficantly decreased compared with control group in the cardiac tissues.The decreased number, morphological abnormalities such as dissolved cavitation, matrix and obvious tumefaction of mitochondria were observed under transmission electron mi-croscope.The microwave radiation induced the apoptosis of myocardial cells in the rats.The cell apoptotic rate and the pro-tein level of cleaved caspase-3 increased with increasing intensity of microwave radiation ( P<0.05 ) .CONCLUSION:Microwave radiation has obvious injury effect on the rat heart, which can cause cardiac energy metabolism dysregulation and cardiac myocyte apoptosis.
