Cigarette smoke extract promotes senescence by reducing expression of HDAC2 in C2C12 myoblasts
10.3969/j.issn.1000-4718.2015.04.029
- VernacularTitle:香烟提取物通过减少 HDAC2表达诱导小鼠 C2 C12成肌细胞衰老
- Author:
Wenyan SU
;
Wenting LIU
;
Xia YANG
;
Jing BAI
;
Zhiyi HE
- Publication Type:Journal Article
- Keywords:
Cigarette smoke extract;
C2C12 myoblasts;
Senescence;
Histone deacetylase 2
- From:
Chinese Journal of Pathophysiology
2015;33(4):738-743
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate whether the cigarette smoke extract (CSE) causes senescence of C2C12 myo-blasts and the relationship between senescence and histone deacetylase 2(HDAC2).METHODS: Murine C2C12 cells were induced to differentiate into myoblasts.The HDAC2 activator and inhibitor were used to investigate the effects of CSE in the myoblasts on cell senescence and the expression of HDAC2.The expression of HDAC2 at mRNA and protein levels was determined by real-time PCR and Western blot, respectively, and the positive cell rate of β-galactosidase staining for cell senescence was also detected.RESULTS:The optimal concentration of CSE was 60 mL/L and the intervention time was 24 h.After the intervention of CSE, the positive cell rate ofβ-galactosidase staining was increased, accompanied with the reduction of HDAC2 expression at mRNA and protein levels.The expression of HDAC2 at mRNA and protein levels was increased by 4, 5, 6, 7-tetrabromobenzotriazole (TBB), accompanied with the reduction of positive cell rate ofβ-galacto-sidase staining.Furthermore, when HDAC2 expression at mRNA and protein levels was reduced by HDAC2 inhibitor valp-roic acid, the positive cell rate of β-galactosidase staining was increased.CONCLUSION: CSE promotes the senescence by reducing the expression of HDAC2 in C2C12 myoblasts.
