Effect of DEC1 gene over-expression on proliferation and invasion abili-ties of human esophageal cancer ECA109 cells
10.3969/j.issn.1000-4718.2015.04.008
- VernacularTitle:DEC1基因过表达对人食管癌 ECA109细胞增殖及侵袭能力的影响
- Author:
Chunping YANG
;
Huachuan WANG
;
Jianhu WEN
- Publication Type:Journal Article
- Keywords:
DEC1 gene;
Esophageal cancer;
ECA109 cells;
Cell proliferation;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2015;33(4):620-624
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effect of DEC1 gene over-expression on the proliferation and invasion abili-ties of human esophageal cancer ECA109 cells.METHODS: ECA109 cells were transfected with plasmid pcDNA3.1 (-)/DEC1 (DEC1 group) or pcDNA3.1 (-) (vector group).The mRNA and protein levels of DEC1, cyclin D1 and MMP-9 were evaluated by real-time PCR and Western blot, respectively.The effects of DEC1 over-expression on the prolif-eration and invasion abilities of the ECA109 cells were evaluated by CCK-8 assay, colony formation assay and Transwell test respectively.RESULTS:The DEC1 expression level in ECA109 cells in DEC1 group was significantly higher than that in vector group (P<0.01), but the levels of MMP9 and cyclin D1 expression were opposite (P<0.01).However, both the proliferation and invasion abilities of ECA109 cells in DEC1 groups decreased significantly as compared with those in vector group (P<0.05).CONCLUSION:The over-expression of DEC1 significantly inhibits the proliferation and invasion of ECA109 cells, which may be involved in the expression of cyclin D1 and MMP9.
