Effect of monocyte-secreted VEGF induced by electrical burn serum on monocyte-endothelial cell adhesion
10.3969/j.issn.1000-4718.2015.04.032
- VernacularTitle:电烧伤大鼠血清诱导单核细胞分泌 VEGF对单核-内皮细胞黏附作用的影响
- Author:
Qiongfang RUAN
;
Chaoli ZHAO
;
Ziqing YE
;
Qionghui XIE
;
Weiguo XIE
- Publication Type:Journal Article
- Keywords:
Electrical burns;
Monocytes;
Endothelial cells;
Adhesion
- From:
Chinese Journal of Pathophysiology
2015;33(4):755-758
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the level of vascular endothelial growth factor (VEGF) secreted by monocytes cultured with electrical burn serum, and to explore the effect of VEGF on monocyte-endothelial cell adhesion.METH-ODS:The electrical burn serum of the rat was prepared.The normal serum from the rats without treating electric current was also collected for control.The contents of VEGF and its soluble receptor sFlt-1 in electrical burn group were determined by double-antibody sandwich ELISA.THP-1 cells were randomly divided into normal serum group and electrical burn serum group.The contents of VEGF and sFlt-1 in the culture supernatants were measured by double-antibody sandwich ELISA. THP-1 cells were also randomly divided into another 4 groups:normal serum group, electrical burn serum group, normal serum +inhibitor group and electrical burn serum +inhibitor group.THP-1 cells, which were incubated with the serum for 3 h and 6 h, were labeled with calcein-AM and then were added into the well with monolayer of endothelial cell line EA.hy926 to detect monocyte-endothelial cell adhesion.RESULTS:The levels of serum VEGF of the rats with electrical burns were significantly increased, the levels of serum sFlt-1 were significantly decreased as compared with the controls. The levels of VEGF secreted by THP-1 cells cultured with electrical burn serum were significantly increased, the levels of sFlt-1 were decreased correspondingly.Electrical burn serum enhanced monocyte-endothelial cell adhesion, sFlt-1 inhibi-ted the adhesion between monocytes and endothelial cells.CONCLUSION:The monocytes exposed to the electrical burn serum secrete VEGF, which enhance the adhesion between monocytes and endothelial cells.Blockage of VEGF activity may effectively inhibit monocyte-endothelial cell adhesion.
