Periodontal tissue remodeling assisted by three kinds of orthodontic appliances: osteoclast differentiation factor mRNA expression under pressure
10.3969/j.issn.2095-4344.2015.16.019
- VernacularTitle:3种不同矫治器正畸作用下牙周骨组织重塑:压力侧破骨细胞分化因子的表达
- Author:
Yan HUANG
;
Na GUO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(16):2562-2566
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Recent studies have shown that osteoclast differentiation factor is closely related to osteoclast differentiation, formation and function in bone remodeling during orthodontic tooth movement. OBJECTIVE:To observe the effects of three kinds of orthodontic appliances on the expression of osteoclast differentiation factor at the pressure side of rat periodontal tissue during remodeling process and to explore the biocompatibility of the orthodontic appliances with the host tissues during orthodontic treatment. METHODS:Eighty healthy Wistar rats were selected to establish animal models of orthodontic tooth movement, and then randomly divided into four groups: control group, MBT group, Begg group, Damon Ⅲ appliance group. Four animal from each group were sacrificed at 3, 7, 14 days after wearing orthodontic appliances. The tartrate-resistant acid phosphatase staining was used to count the osteoclasts at the pressure side of alveolar bone tissue; real-time quantitative PCR detection to detect mRNA expression of osteoclast differentiation factor at the pressure side of periodontal tissue and time distribution characteristics. RESULTS AND CONCLUSION:Compared with the control group, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were increased with orthodontic time, reached the peaked at 7 days and then gradualy decreased. The number of positive osteoclastsand mRNA expression of osteoclast differentiation factor at the pressure side of the alveolar bone tissue were significantly higher in the Damon Ⅲ group than the other three groups at 7 days after orthodontic treatment (P < 0.05). These findings indicate that, during the bone remodeling, the number of positive osteoclasts changed in accordance with the mRNA expression of osteoclast differentiation factor, and at 7 days, the number of positive osteoclasts and mRNA expression of osteoclast differentiation factor were highest in the Damon Ⅲ group.
