Toxicity of blastula culture medium to mouse embryos: a safety evaluation ofinvitro assisted reproduction devices
10.3969/j.issn.2095-4344.2015.16.026
- VernacularTitle:囊胚培养液对小鼠胚胎的毒性:体外生殖辅助医疗器械安全性评价
- Author:
Qianqian HAN
;
Yanyun YIN
;
Han WANG
;
Xiaoming FENG
;
Chunren WANG
;
Zhaopeng YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(16):2598-2602
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The blastula culture medium can assist the development of zygote from the fertilized egg to the blast blastula. The safety and quality of blastula culture medium directly influences the quality of blastula. OBJECTIVE:To evaluate the effect of blastula culture medium on the development of mouse embryos. METHODS: In this study, B6D2F1 mice were used. The female mice were superovulated and mated with male B6D2F1 mice. One day later, the zygotes were colected and cultured in the M16 medium to 4-cel stage. Then, 4-cel stage embryos were transferred into the tested blastula culture medium (experimental group). After 5 days of culture, the forming rate of blastula was examined. Meanwhile, the M16 medium containing endotoxin was used to culture 1-cel mouse zygote (positive control group). The M16 medium with no embryo toxicity was used to culture 1-cel zygote (negative control group). RESULTS AND CONCLUSION:The formation rate of blastula was 0 in the positive group, 87.1% in the negative control group, and 87.3% in the experimental group. From the results, the tested blastula culture medium could assist the 1-cel zygote growing to the stage of blastula, and the formation rate of blastula was above 80%. The tested blastula culture medium had no toxicity to the mouse embryo.
