Study macroporous adsorptive resins on purification technology of polysaccharides from Lonicera japonica Thunb
10.3760/cma.j.issn.1673-4246.2015.04.015
- VernacularTitle:大孔吸附树脂对金银花多糖脱色工艺研究
- Author:
Zhihao LI
;
Yinhua CHEN
;
Peng LI
;
Fang ZHENG
;
Jun ZHU
;
Linjie HUANG
;
Cong LI
- Publication Type:Journal Article
- Keywords:
Lonicera japonica Thunb.;
Macroporous adsorptive resins;
Decoloration
- From:
International Journal of Traditional Chinese Medicine
2015;(4):345-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of macroporous adsorptive resins on the decoloration technology ofLonicera japonica Thunb. polysaccharides(FLP).Methods The effects of 6 kinds of macroporous adsorptive resins i.e. HPD-400A, AB-8, HPD-750, HPD-100, D3520, D301T, S8 on the decolorization technology ofLonicera japonica Thunb. polysaccharides were compared with single factor test in terms of temperature, polysaccharide concentration, pH, adsorption flow, and eluant.Results The decolorization rate and polysaccharide retention rate of S8 were optimal. The best decoloration conditions were as follows: temperature of 40℃, polysaccharide concentration of 5 mg/ml, pH value of 6, flow rate of 1 ml/min, distilled water with pH=6 as eluant. The adsorption rate was 83.2%,and polysaccharide retention rate was 72.1%.Conclusion High decolorization ratio and the high retention rate ofLonicera japonica Thunb. could be obtained by means of decoloration with S8 macroporous adsorptive resins..
