Effect of miR-145 on NSCLC cell proliferation and FSCN1 protein expression
- VernacularTitle:miR－145对NSCLC细胞增殖和FSCN1蛋白表达的影响研究
- Author:
Zhimin MU
;
Guangxian MAO
;
Yuancai XIE
;
Xuxing PENG
;
Da WU
- Publication Type:Journal Article
- Keywords:
miR-145;
fascinhomolog1;
cell proliferation
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(3):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-145 on human lung adenocarcinaoma A549 cell proliferation and FSCN1 expression. Methods pmR-mcherry/miR-145 was constructed and transfected into A549 cell,then the expression of miR-145 and the proliferation of A549 cell were verified by QPCR and MTS assay, respectively.The situation of FSCN1 expression in A549 cell was detected by Western blot.Results pmR-mcherry/miR-145 vector was constructed successfully,and QPCR results indicated that miR-145 expressed effectively.Western blot results showed that FSCN1 was one of the targets of miR-145 in A549 cell.MTS assay results indicated that miR-145 inhibited the proliferation of A549 cell.Conclusion Overexpression of miR-145 can inhibit the proliferation of A549 cell, and FSCN1 was one of its target.
