Study on oxidative cutting DNA with pentanuclear copper complexes of imino acetic acid two aromatic derivatives
- VernacularTitle:亚氨基二乙酸芳香衍生物的多核铜配合物氧化切割DNA研究
- Author:
Linlin WU
;
Meng LI
;
Ting HUANG
- Publication Type:Journal Article
- Keywords:
pentanuclear copper complexes;
DNA oxidative cutting;
artificial metallonucleases
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(3):72-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effect of pentanuclear copper complexes of imino acetic acid two aromatic derivatives on oxidative cutting DNA. Methods Synthesized complexes later, protonation constants and the stability constants of the complexes were determined by potentiometric titration method, the cutting DNA was studied by agarose gelelectrophoresis.Results Under physiological conditions, complexes H4L1, H4L2, between H6L3 and Cu2 +coordination capability was stronger; three carboxylic multi copper complexes in Cu2 + concentration cutting efficiency were higher than the corresponding mononuclear copper complexes, synergistic effect exists between Cu2 + Center; H6L3 complexes of the three DNA cut the highest efficiency, complex H4L1 was followed, complex H4L2 cutting efficiency was the lowest.Conclusion Change the complex chelating ligands has great influence on the properties of complex, which provides a theoretical basis for the design and synthesis of copper nucleases reasonable.
