The Effect of Gabapentin on TRPV1 in Rat DRG after CCI
- VernacularTitle:加巴喷丁对大鼠坐骨神经慢性压迫损伤DRG中TRPV1的影响
- Author:
Ni YAN
;
Yong LUAN
;
Runjiu LI
- Publication Type:Journal Article
- Keywords:
neuralgia;
Gabapentin;
chronic constriction injury;
rat;
TRPV1
- From:
Journal of China Medical University
2015;(3):234-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Gabapentin(GBP)on the transient receptor potential vanilloid l(TRPV1)in dorsal root ganglion (DRG)of rats with chronic constriction injury(CCI). Methods SD male rats were used to establish CCI model and randomly divided into 3 groups,the control group,the CCI group,and the CCI+GBP group. Mechanical allodynia and thermal hyperalgesia were measured to evaluate the es?tablishment of CCI model and the analgesic effect of GBP. At the postoperative 8 days,all rats were sacrificed and dorsal root ganglions were collect?ed for further RT?PCR and Western?blotting analysis to detect expression of TRVP1 in DRG. Results The mRNA and protein expression of TRPV1 were both significantly increased in DRG after CCI operation and GBP could decrease mRNA expression of TRPV1 gene in DRG after CCI. Conclusion The expression of TRPV1 was increased in DRG after CCI operation which was decreased after administration of GBP.
