Preparation and application on hepatitis B surface antisen feeble masculine serum
10.3969/j.issn.1673-4130.2015.14.017
- VernacularTitle:乙肝表面抗原室内弱阳性质控物的制备及其应用评价
- Author:
Yun LIU
;
Lingjuan GAO
- Publication Type:Journal Article
- Keywords:
hepatitis B surface antisen;
serum showing feeble masculine;
preparation
- From:
International Journal of Laboratory Medicine
2015;(14):1994-1995
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .
